NCT07219498 · University of Minnesota
Effects of a 5-Week Probiotic Supplementation Intervention on Markers of Delayed Onset Muscle Soreness
What this study is about
The purpose of this study is to evaluate how probiotic supplementation (PS) influences post-exercise recovery in healthy, physically active young adults.
View original scientific description
The purpose of this study is to evaluate how probiotic supplementation (PS) influences post-exercise recovery in healthy, physically active young adults. Researchers aim to determine if the Lactobacillus casei probiotic strain reduces symptoms of delayed onset muscle soreness (DOMS) based on perceived muscle soreness, pressure pain threshold, and power output following exercise induced muscle damage (EIMD).
Interventions
DIETARY_SUPPLEMENT
Lactobacillus casei
Probiohealth is the manufacturer of this strain and they use a stringent quality control program that utilizes a multi point product variability check system.
OTHER
Placebo Capsule(s)
The brand name of placebo capsules that will be used in this study is Magic Bullet Placebo Capsules. This placebo is manufactured by Progressive Placebo as stated on the product label.
Primary outcome measures
Change in perceived muscle soreness
Time frame: From baseline to post 5-week intervention
Visual Analog Scale for perceived muscle soreness Each number on the scale will be designated with a descriptive word that corresponds to the soreness level they are experiencing. The scale will be laid out in order of increasing muscle soreness with "1" representing no pain.
Change in pressure pain threshold (PPT)
Time frame: From baseline to post 5-week intervention
Calf pressure pain threshold (PPT) will be assessed by placing marks on the medial condyle of the femur and the posterior of the medial malleolus. The researcher will place the algometer on the muscle belly exactly halfway between the two marks. Volunteers will be instructed to specify when the first onset of pain occurs as the researcher gradually applies pressure. Quadricep measurements will involve the participant lying in the supine position where marks will be made on the anterior inferior iliac spine and the top of the patella so the researchers can once again find a point exactly halfway between these two locations to apply the algometer. Participants will be instructed to verbally indicate to the researcher when the onset of pain occurs as pressure is applied.
Change in countermovement jump assessment (CMJ) performance recovery
Time frame: From baseline to post 5-week intervention
The researchers will demonstrate and instruct volunteers to "Stand upright with their hands above their head. When you are ready, bend your knees and stick your hips back while keeping your hands to your hips. Jump as high as possible and land softly." Participants will perform three CMJs separated by 60 seconds. Jump height will be recorded in cm and the highest value will be recorded.
Who can participate
This study lists these criteria on ClinicalTrials.gov. A study coordinator reviews eligibility during screening — this page does not determine whether you qualify.
Inclusion criteria
- complete a minimum of 150 minutes a week of at least moderate intensity exercise in either an endurance-based or resistance-based training style based on a self-reported physical activity over the past month
- good general health with no injuries or health conditions that would affect physical activity
- willing to abstain from consistent NSAID use and other probiotic supplements over the course of this study
Exclusion criteria
- known musculoskeletal injuries or physical limitations that could interfere with performing the designated physical activity required
- underlying conditions that could place them at an increased risk during exercise such as cardiovascular, respiratory, or metabolic diseases
- currently take a probiotic supplement, are prescribed and taking an antibiotic during the intervention, or report using NSAIDs or steroids within the recovery time window
- report using an NSAID more than twice a week on a regular basis
Where
- Minneapolis, Minnesota
Related conditions & keywords
Frequently asked questions
What is a clinical trial?
A clinical trial is a research study that tests new medical treatments, drugs, devices, or procedures to determine their safety and effectiveness. Trials are carefully designed and monitored to protect participants while advancing medical knowledge.
Is it safe to participate?
Clinical trials follow strict safety guidelines and ethical standards. Trials must be reviewed and approved, and participants are closely monitored by medical professionals throughout the study. You can withdraw at any time if you choose.
Will I be compensated?
Many clinical trials offer compensation for your time, travel expenses, and inconvenience. The specific compensation varies by study and will be discussed during the screening process. All study-related medical care is typically provided at no cost to participants.
Will I receive a placebo instead of treatment?
When effective treatment exists, participants typically receive either the standard treatment plus the study intervention, or the standard treatment plus placebo. You would not be denied effective care. Placebos are primarily used when no proven treatment is available, or in addition to standard care. Your trial consent form will clearly explain what treatments you may receive.
Can I leave a trial if I change my mind?
Absolutely. Participation in clinical trials is completely voluntary. You have the right to withdraw from the study at any time, for any reason, without penalty or loss of benefits to which you are otherwise entitled.
How long does a clinical trial last?
Trial duration varies widely depending on the study design and purpose. Some trials last just a few weeks, while others may continue for months or years. The study coordinator will provide specific timeline information during your screening call.
Data: ClinicalTrials.gov · synced May 4, 2026 · Source of record for eligibility and locations